LOCUS NM_001400620 6617 bp mRNA linear PRI 22-MAR-2023 DEFINITION Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 27, mRNA. ACCESSION NM_001400620 VERSION NM_001400620.1 KEYWORDS RefSeq. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 6617) AUTHORS Chen Q, Zheng X, Lin Q and Chen J. TITLE Retrospective Analysis of the Expression of CD56 in Multiple Myeloma with Bone-Related Extramedullary Diseases JOURNAL Ann Clin Lab Sci 53 (1), 14-20 (2023) PUBMED 36889774 REMARK GeneRIF: Retrospective Analysis of the Expression of CD56 in Multiple Myeloma with Bone-Related Extramedullary Diseases. Review article REFERENCE 2 (bases 1 to 6617) AUTHORS Liang T, Peng Z, Li C, Huang J, Wang H, Bu C, Li J, Zheng Y, Feng X, Li H and Chen C. TITLE Evaluating the prognostic value of CD56 in pediatric acute myeloid leukemia JOURNAL BMC Cancer 22 (1), 1339 (2022) PUBMED 36544113 REMARK GeneRIF: Evaluating the prognostic value of CD56 in pediatric acute myeloid leukemia. Publication Status: Online-Only REFERENCE 3 (bases 1 to 6617) AUTHORS Seymour F, Cavenagh JD, Mathews J and Gribben JG. TITLE NK cells CD56bright and CD56dim subset cytokine loss and exhaustion is associated with impaired survival in myeloma JOURNAL Blood Adv 6 (17), 5152-5159 (2022) PUBMED 35834731 REMARK GeneRIF: NK cells CD56bright and CD56dim subset cytokine loss and exhaustion is associated with impaired survival in myeloma. REFERENCE 4 (bases 1 to 6617) AUTHORS Picard LK, Claus M, Fasbender F and Watzl C. TITLE Human NK cells responses are enhanced by CD56 engagement JOURNAL Eur J Immunol 52 (9), 1441-1451 (2022) PUBMED 35775327 REMARK GeneRIF: Human NK cells responses are enhanced by CD56 engagement. REFERENCE 5 (bases 1 to 6617) AUTHORS Cottini F, Rodriguez J, Hughes T, Sharma N, Guo L, Lozanski G, Liu B, Cocucci E, Yang Y and Benson D. TITLE Redefining CD56 as a Biomarker and Therapeutic Target in Multiple Myeloma JOURNAL Mol Cancer Res 20 (7), 1083-1095 (2022) PUBMED 35380709 REMARK GeneRIF: Redefining CD56 as a Biomarker and Therapeutic Target in Multiple Myeloma. REFERENCE 6 (bases 1 to 6617) AUTHORS Rossell RJ, Malik A, Helfrich W, Kemshead JT and Patel K. TITLE A discordance between the human muscle NCAM sequence and those seen in other NCAM cDNA clones JOURNAL Biochim Biophys Acta 1130 (1), 95-96 (1992) PUBMED 1543753 REFERENCE 7 (bases 1 to 6617) AUTHORS van Duijnhoven HL, Helfrich W, de Leij L, Roebroek AJ, van de Ven WJ, Healey K, Culverwell A, Rossell RJ, Kemshead JT and Patel K. TITLE Splicing of the VASE exon of neural cell adhesion molecule (NCAM) in human small-cell lung carcinoma (SCLC) JOURNAL Int J Cancer 50 (1), 118-123 (1992) PUBMED 1339414 REFERENCE 8 (bases 1 to 6617) AUTHORS Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ and Phillips JH. TITLE Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56) JOURNAL J Immunol 146 (12), 4421-4426 (1991) PUBMED 1710251 REFERENCE 9 (bases 1 to 6617) AUTHORS Bello MJ, Salagnon N, Rey JA, Guichaoua MR, Berge-Lefranc JL, Jordan BR and Luciani JM. TITLE Precise in situ localization of NCAM, ETS1, and D11S29 on human meiotic chromosomes JOURNAL Cytogenet Cell Genet 52 (1-2), 7-10 (1989) PUBMED 2612216 REFERENCE 10 (bases 1 to 6617) AUTHORS Dickson,G., Gower,H.J., Barton,C.H., Prentice,H.M., Elsom,V.L., Moore,S.E., Cox,R.D., Quinn,C., Putt,W. and Walsh,F.S. TITLE Human muscle neural cell adhesion molecule (N-CAM): identification of a muscle-specific sequence in the extracellular domain JOURNAL Cell 50 (7), 1119-1130 (1987) PUBMED 2887295 COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FP884230.1, AP002853.4 and AP000880.4. Summary: This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein plays a role in the development of the nervous system by regulating neurogenesis, neurite outgrowth, and cell migration. This protein is also involved in the expansion of T lymphocytes, B lymphocytes and natural killer (NK) cells which play an important role in immune surveillance. This protein plays a role in signal transduction by interacting with fibroblast growth factor receptors, N-cadherin and other components of the extracellular matrix and by triggering signalling cascades involving FYN-focal adhesion kinase (FAK), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3K). One prominent isoform of this gene, cell surface molecule CD56, plays a role in several myeloproliferative disorders such as acute myeloid leukemia and differential expression of this gene is associated with differential disease progression. For example, increased expression of CD56 is correlated with lower survival in acute myeloid leukemia patients whereas increased severity of COVID-19 is correlated with decreased abundance of CD56-expressing NK cells in peripheral blood. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Aug 2020]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## coronavirus related :: involved in immune response or antiviral activity ##RefSeq-Attributes-END## PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-245 FP884230.1 13-257 246-320 AP002853.4 147810-147884 321-539 AP002853.4 149717-149935 540-683 AP002853.4 150954-151097 684-821 AP002853.4 151474-151611 822-939 AP002853.4 152692-152809 940-1109 AP002853.4 153264-153433 1110-1252 AP000880.4 2844-2986 1253-1403 AP000880.4 20120-20270 1404-1588 AP000880.4 20645-20829 1589-1685 AP000880.4 21193-21289 1686-1856 AP000880.4 21622-21792 1857-1988 AP000880.4 23508-23639 1989-2003 AP000880.4 24776-24790 2004-2128 AP000880.4 44352-44476 2129-2306 AP000880.4 48621-48798 2307-2514 AP000880.4 58663-58870 2515-2631 AP000880.4 60235-60351 2632-3447 AP000880.4 61408-62223 3448-6617 AP000880.4 63742-66911 FEATURES Location/Qualifiers source 1..6617 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /chromosome="11" /map="11q23.2" gene 1..6617 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="neural cell adhesion molecule 1" /db_xref="GeneID:4684" /db_xref="HGNC:HGNC:7656" /db_xref="MIM:116930" exon 1..245 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" CDS 194..3568 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /EC_number="2.7.11.1" /note="isoform 27 precursor is encoded by transcript variant 27; antigen recognized by monoclonal antibody 5.1H11; neural cell adhesion molecule, NCAM" /codon_start=1 /product="neural cell adhesion molecule 1 isoform 27 precursor" /protein_id="NP_001387549.1" /db_xref="GeneID:4684" /db_xref="HGNC:HGNC:7656" /db_xref="MIM:116930" /translation="MLQTKDLIWTLFFLGTAVSLQVDIVPSQGEISVGESKFFLCQVA GDAKDKDISWFSPNGEKLTPNQQRISVVWNDDSSSTLTIYNANIDDAGIYKCVVTGED GSESEATVNVKIFQKLMFKNAPTPQEFREGEDAVIVCDVVSSLPPTIIWKHKGRDVIL KKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTIQARQ NIVNATANLGQSVTLVCDAEGFPEPTMSWTKDGEQIEQEEDDEKYIFSDDSSQLTIKK VDKNDEAEYICIAENKAGEQDATIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGD PIPSITWRTSTRNISSEEKTLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIG QDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNY SNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDQV EPYSSTAQVQFDEPEATGGVPILKYKAEWRAVGEEVWHSKWYDAKEASMEGIVTIVGL KPETTYAVRLAALNGKGLGEISAASEFKTQPVHSPPPREPSAPKLEGQMGEDGNSIKV NLIKQDDGGSPIRHYLVRYRALSSEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAEN QQGKSKAAHFVFRTSAQPTAIPANGSPTSGLSTGAIVGILIVIFVLLLVVVDITCYFL NKCGLFMCIAVNLCGKAGPGAKGKDMEEGKAAFSKDESKEPIVEVRTEEERTPNHDGG KHTEPNETTPLTEPELPADTTATVEDMLPSVTTVTTNSDTITETFATAQNSPTSETTT LTSSIAPPATATPDSNSVPAGQATPSKGPSASAPSPAPASAPKVAPLVDLSDTPTSTP AASNLSSSVLANQGAVLSPSAPAGVGEASKAPPASKPTPAPVPTPTGAASPLAAAAAP ATEAPQAKQEAPSTKGPDPEPTQPGAAKSPAEAATALASPKSEAASVSTTNPSQGEDF KMDEGNFKTPDIDLAKDVFAALGSPAPAAGASGQAPELAPSTADSSVSPAPAKTEKGP VEAKPECQETETKPAPAEVKTVPNDATQTKENESKA" sig_peptide 194..250 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="COORDINATES: ab initio prediction:SignalP:4.0" misc_feature 857..859 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="N-linked (GlcNAc...) asparagine. /evidence=ECO:0000255; propagated from UniProtKB/Swiss-Prot (P13591.3); glycosylation site" misc_feature 1136..1138 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="N-linked (GlcNAc...) asparagine. /evidence=ECO:0000255; propagated from UniProtKB/Swiss-Prot (P13591.3); glycosylation site" misc_feature 1232..1234 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="N-linked (GlcNAc...) asparagine. /evidence=ECO:0000255; propagated from UniProtKB/Swiss-Prot (P13591.3); glycosylation site" misc_feature 1460..1462 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="N-linked (GlcNAc...) asparagine. /evidence=ECO:0000255; propagated from UniProtKB/Swiss-Prot (P13591.3); glycosylation site" misc_feature 1538..1540 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="N-linked (GlcNAc...) asparagine. /evidence=ECO:0000269|PubMed:16335952; propagated from UniProtKB/Swiss-Prot (P13591.3); glycosylation site" misc_feature 1625..1627 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="N-linked (GlcNAc...) asparagine. /evidence=ECO:0000255; propagated from UniProtKB/Swiss-Prot (P13591.3); glycosylation site" misc_feature 2330..2392 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="propagated from UniProtKB/Swiss-Prot (P13591.3); transmembrane region" misc_feature 2513..2515 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="Phosphoserine. /evidence=ECO:0000250|UniProtKB:P13595; propagated from UniProtKB/Swiss-Prot (P13591.3); phosphorylation site" misc_feature 2525..2527 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="Phosphoserine. /evidence=ECO:0000250|UniProtKB:P13595; propagated from UniProtKB/Swiss-Prot (P13591.3); phosphorylation site" exon 246..320 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 321..539 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 540..683 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 684..821 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 822..939 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 940..1109 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 1110..1252 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 1253..1403 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 1404..1588 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 1589..1685 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 1686..1856 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 1857..1988 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 1989..2003 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 2004..2128 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 2129..2306 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 2307..2514 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 2515..2631 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 2632..3447 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" exon 3448..6617 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /inference="alignment:Splign:2.1.0" regulatory 4761..4766 /regulatory_class="polyA_signal_sequence" /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="hexamer: AATAAA" polyA_site 4780 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="major polyA site" regulatory 6593..6598 /regulatory_class="polyA_signal_sequence" /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="hexamer: AATAAA" polyA_site 6617 /gene="NCAM1" /gene_synonym="CD56; MSK39; NCAM" /note="major polyA site" ORIGIN 1 actcattctc cgatcagcgc gtgaacgcag ctcggctgcc gctggcagga aacaattctg 61 caaaaataat catactcagc ctggcaattg tctgccccta ggtctgtcgc tcagccgccg 121 tccacactcg ctgcaggggg gggggcacag aatttaccgc ggcaagaaca tccctcccag 181 ccagcagatt acaatgctgc aaactaagga tctcatctgg actttgtttt tcctgggaac 241 tgcagtttct ctgcaggtgg atattgttcc cagccagggg gagatcagcg ttggagagtc 301 caaattcttc ttatgccaag tggcaggaga tgccaaagat aaagacatct cctggttctc 361 ccccaatgga gaaaagctca ccccaaacca gcagcggatc tcagtggtgt ggaatgatga 421 ttcctcctcc accctcacca tctataacgc caacatcgac gacgccggca tttacaagtg 481 tgtggttaca ggcgaggatg gcagtgagtc agaggccacc gtcaacgtga agatctttca 541 gaagctcatg ttcaagaatg cgccaacccc acaggagttc cgggaggggg aagatgccgt 601 gattgtgtgt gatgtggtca gctccctccc accaaccatc atctggaaac acaaaggccg 661 agatgtcatc ctgaaaaaag atgtccgatt catagtcctg tccaacaact acctgcagat 721 ccggggcatc aagaaaacag atgagggcac ttatcgctgt gagggcagaa tcctggcacg 781 gggggagatc aacttcaagg acattcaggt cattgtgaat gtgccaccta ccatccaggc 841 caggcagaat attgtgaatg ccaccgccaa cctcggccag tccgtcaccc tggtgtgcga 901 tgccgaaggc ttcccagagc ccaccatgag ctggacaaag gatggggaac agatagagca 961 agaggaagac gatgagaagt acatcttcag cgacgatagt tcccagctga ccatcaaaaa 1021 ggtggataag aacgacgagg ctgagtacat ctgcattgct gagaacaagg ctggcgagca 1081 ggatgcgacc atccacctca aagtctttgc aaaacccaaa atcacatatg tagagaacca 1141 gactgccatg gaattagagg agcaggtcac tcttacctgt gaagcctccg gagaccccat 1201 tccctccatc acctggagga cttctacccg gaacatcagc agcgaagaaa agactctgga 1261 tgggcacatg gtggtgcgta gccatgcccg tgtgtcgtcg ctgaccctga agagcatcca 1321 gtacactgat gccggagagt acatctgcac cgccagcaac accatcggcc aggactccca 1381 gtccatgtac cttgaagtgc aatatgcccc aaagctacag ggccctgtgg ctgtgtacac 1441 ttgggagggg aaccaggtga acatcacctg cgaggtattt gcctatccca gtgccacgat 1501 ctcatggttt cgggatggcc agctgctgcc aagctccaat tacagcaata tcaagatcta 1561 caacaccccc tctgccagct atctggaggt gaccccagac tctgagaatg attttgggaa 1621 ctacaactgt actgcagtga accgcattgg gcaggagtcc ttggaattca tccttgttca 1681 agcagacacc ccctcttcac catccatcga ccaggtggag ccatactcca gcacagccca 1741 ggtgcagttt gatgaaccag aggccacagg tggggtgccc atcctcaaat acaaagctga 1801 gtggagagca gttggtgaag aagtatggca ttccaagtgg tatgatgcca aggaagccag 1861 catggagggc atcgtcacca tcgtgggcct gaagcccgaa acaacgtacg ccgtaaggct 1921 ggcggcgctc aatggcaaag ggctgggtga gatcagcgcg gcctccgagt tcaagacgca 1981 gccagtccat agccctcctc cacgggaacc cagtgcacct aagctcgaag ggcagatggg 2041 agaggatgga aactctatta aagtgaacct gatcaagcag gatgacggcg gctcccccat 2101 cagacactat ctggtcaggt accgagcgct ctcctccgag tggaaaccag agatcaggct 2161 cccgtctggc agtgaccacg tcatgctgaa gtccctggac tggaatgctg agtatgaggt 2221 ctacgtggtg gctgagaacc agcaaggaaa atccaaggcg gctcattttg tgttcaggac 2281 ctcggcccag cccacagcca tcccagccaa cggcagcccc acctcaggcc tgagcaccgg 2341 ggccatcgtg ggcatcctca tcgtcatctt cgtcctgctc ctggtggttg tggacatcac 2401 ctgctacttc ctgaacaagt gtggcctgtt catgtgcatt gcggtcaacc tgtgtggaaa 2461 agccgggccc ggggccaagg gcaaggacat ggaggagggc aaggccgcct tctcgaaaga 2521 tgagtccaag gagcccatcg tggaggttcg aacggaggag gagaggaccc caaaccatga 2581 tggagggaaa cacacagagc ccaacgagac cacgccactg acggagcccg agctgcctgc 2641 cgacactacg gccactgtcg aggacatgct gccttctgtc accaccgtca ccactaactc 2701 tgacactatc accgaaacct ttgccactgc tcagaacagc cccaccagtg agaccaccac 2761 cctgacctcc agtattgccc cgccggccac ggccacgcct gactcaaact ctgtaccggc 2821 tggccaggcc accccttcca aggggcccag cgcctctgcc ccctccccgg ccccagcttc 2881 agcccccaag gtcgcccccc tcgttgacct gagcgacacc ccgacctcaa cccctgccgc 2941 tagcaatttg tcttctagtg tcctggctaa ccaaggggct gtcctcagcc caagcgcccc 3001 tgctggtgtc ggggaggcct ctaaggctcc tccggccagc aagcccaccc ctgcaccagt 3061 ccccaccccg actggggcag ccagtcctct agcagcagcg gctgcccctg ccacagaagc 3121 ccctcaggcc aagcaggagg ctcccagcac caaaggcccg gacccggagc ccacccagcc 3181 cggagccgcg aagagcccgg ccgaggcagc cacagccctt gctagcccga agagcgaggc 3241 tgcctccgtc agcaccacaa acccttccca gggcgaggac tttaaaatgg acgaagggaa 3301 cttcaagacc ccagatattg accttgcaaa ggatgttttt gcagccctgg gctctcctgc 3361 tcccgccgct ggggccagtg gacaagcccc tgagcttgct ccttccactg cagacagctc 3421 tgtttcgcct gcgccagcaa agaccgagaa gggccccgta gaagcaaagc cagagtgcca 3481 ggagacagaa acgaagccag cgccagccga agtcaagacg gtccccaatg acgccacaca 3541 gacaaaggag aacgagagca aagcatgatg ggtgaagaga accgagcaaa gatcaaaata 3601 aaaagtgaca cagcagcttc accagagcat ttccaacacc acagacacac acacgcacgc 3661 acacacacaa acacacatgc acacacacac atctcatttc tctagtgtct tttgccttta 3721 aaaaaaacta aacagataaa acatgggaat ctcctttttg taggtttata gaaagggtcc 3781 ctttgttgca cactcacttg taagaaaatg agacaaaaag gttaaaccca cagccaaact 3841 aggacactcc gttccctgaa accgttaaaa aatcaaacaa aaggacccca aattaagaat 3901 ctaggaagct cagaaacgaa atctaggttc aggaagacca cacttggtgt tacccgattg 3961 gcacagacca gtttcagaga aatactttca ggcactaaga ctaatcgaat gaacaaagtc 4021 cacagtttat ttttatactt tcagtcaagt ttgaactctg taaaacctca taaataagtt 4081 ataatttctg ttcactttgt atttgttcag tatgcaaagt gtgtcaccct ttctagctga 4141 attcaattcc cacgtagact cttattttat aggacgaatg ccaaattgca gcttctgggg 4201 gtagatctca atttgcagta ttcagacttc tttttctttc ttttacattc ttttttcttt 4261 ctttctttct gccaactttg ttttccagtg tttacaaggt gacaaatgtt tgactttggt 4321 tgtgtttaaa tgtccgtgta aaatagctgc cttttatttt ttaaggtaac aaataccacc 4381 tagaggtagg taggatcatc ccacgcttgc tttagcacag gacaacttta caaaacatga 4441 ttgtttacag ctgctcttcc cctcttttct gatctgcagt ttttgcctgg gtcccactca 4501 ggtgaaaatc catctcattc tggaatggtt ttgcttttga atttttggtt atttttgtgt 4561 ttctttgggg gttagaccac tttctgatta gccgccacct gcctgcatct gtgaaaaggg 4621 atctgctccc aggcgttctc acccttcttt tgaaggactc cttaggcttt gttgaatgaa 4681 gcagagaaga ttgtatagtt ggggctggtc ttggtgaaca cacattatta ccccacacat 4741 cccctttgtg tagaaagcca aataaaatct atacatacca tttccttttg agcccagaat 4801 ctagatttga gcggaagagc atgtgtgctt cagggaatta gtgtcttttt ttggaaatct 4861 gttgaagtaa agtaacatcg gccttctgtt cacttaggca gcatttatag aaacaaaaga 4921 agaaagaaac aacctactgt ctggagtcat aacacaactt tcctggattg gaaaccaagt 4981 gggggaaaaa atacagaaac tttaaggggg atgggagggg ggggagaagg gaaaagccag 5041 ccctttgtat agaaattttg cttttttttc cctcattcta ctttagaact gcaagcttgt 5101 gcactgtgga tgcgtgaata ttttagtgtg aaacgtgttt ttgtcatagt attgaaataa 5161 aacttcaaca tagtttggtt gtggaaggta tagcagatag ttcagaaaaa atattcagga 5221 aacaaaaatc actcaaacgg aatcgaagcc ttttaacaaa gaaaatgaaa tacagatgat 5281 gatgatgatg atgaagatga tgctaagtaa acagaaatca gtactccgca tgcgctcctc 5341 tcctaaggta caaagcagca agaggttagg gtggcaaggc tgcctctggg tccattctgt 5401 gggccactct ccccaacgtt ctgacacttc tgcagtctga tcagtggcga tgctagatta 5461 taatttcaaa ctgtgaagaa taatggtctt gtcatttgct caatgtgggg ttatgttgca 5521 ttttctcagc tcctggggat ggaaatggag gatcccagaa cacacagccc tggccccttt 5581 gattctaggg cctgcacaga tctctggttc aaatgcacag gccctcagaa tagaggaaca 5641 tgaagagaga tcttagagca cacagtagaa tgtgagagcc tgggtgtctg agaccgggag 5701 ggcccagcag tgaggggcag gctcttctgg tcaccaggct gttcagtgga ctcagttctt 5761 catcttgtaa tgtcgatggc tttgccacac caggccaagc ccatgccata ccttgtcaag 5821 actgtcaaag tggttgtggt taggtcaaac tggttttggt tctgatggtt aggaagaaac 5881 aggtcagccc tcagatcacc tggcccggga cagctgaccc cctagaaccc tggctctgcc 5941 attagctagg acctaagact ctgcccacat tttggtctgt tctctcccat tacacatagg 6001 tttgtctcag catgcaagag tttttccttt aaaaaaaaaa aaaaaaaaaa aaaaaaagca 6061 atgctttctc taaaatcaaa gagggagtca ttttattcca agatgtttta tcttttatgt 6121 taagagatca aagcttataa ttttcttttt taatttttga aggagggatc aactccagtt 6181 tccaatgtct atgtgtctat gtgtgtatgt gccatacata tgtattcaca tgaagaccgg 6241 catggccaag ttctgctgga ggagcactca agtgtgacga gcagggccac tggaccctgc 6301 agggctgtgg tgtatatagt gcagctttgg aggtggaact ctattttcac acttttctat 6361 ggagccttcc gagtcccagg ttttcacttg aggctgtctg tctggatggc ggttttcaga 6421 cctccattaa catccctacc cagcattctg tacttcgggg gccttctctc ttgttataaa 6481 actttttacc aagtgaaaca tcgataccac ctttgtttcc attctcactg gtgtaaatac 6541 tgagtactaa ctgagaattt tgactttgca ttctgtcgga atacttgtgt tcaataaaaa 6601 ttgaaagaaa aaagcta //